| 23. kolo |
| 19.04.2023st 15:00 hod.19.04. 15:00 | FK TřinecTRI |  2:1 | FK PříbramPRI | Report |
| 19.04.2023st 17:00 hod.19.04. 17:00 | FK Dukla PrahaDUK |  3:2 | SK Sigma Olomouc BOLB | Report |
| 19.04.2023st 17:00 hod.19.04. 17:00 | SK Slavia Praha BSLA |  1:1 | FC Sellier & Bellot VlašimVLA | Report |
| 19.04.2023st 17:00 hod.19.04. 17:00 | 1.SK ProstějovPRO |  1:0 | MFK VyškovVKV | Report |
| 19.04.2023st 17:00 hod.19.04. 17:00 | MFK KarvináKAR |  2:1 | MFK ChrudimCHR | Report |
| 19.04.2023st 17:00 hod.19.04. 17:00 | FK VarnsdorfVDF |  7:2 | FC Vysočina JihlavaJIH | Report |
| 19.04.2023st 17:00 hod.19.04. 17:00 | SK Líšeň 2019LIS |  3:1 | FC SILON TáborskoTAB | Report |
| 19.04.2023st 17:00 hod.19.04. 17:00 | Slezský FC OpavaSFC |  1:2 | AC Sparta Praha BACS | Report |
